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Quantitating Fluorescence Intensity From Fluorophores: Practical Use of MESF Values

The present work uses fluorescein as the model fluorophore and points out critical steps in the use of MESF (Molecules of Equivalent Soluble Fluorophores) values for quantitative flow cytometric measurements. It has been found that emission spectrum matching between a reference solution and an analy...

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Publicat a:J Res Natl Inst Stand Technol
Autors principals: Wang, Lili, Gaigalas, Adolfas K., Abbasi, Fatima, Marti, Gerald E., Vogt, Robert F., Schwartz, Abe
Format: Artigo
Idioma:Inglês
Publicat: [Gaithersburg, MD] : U.S. Dept. of Commerce, National Institute of Standards and Technology 2002
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4859266/
https://ncbi.nlm.nih.gov/pubmed/27446735
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.6028/jres.107.027
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