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A novel imaging method revealed phosphatidylinositol 3,5-bisphosphate-rich domains in the endosome/lysosome membrane
We developed a new method to observe distribution of phosphatidylinositol 3,5-bisphosphate [PtdIns(3,5)P(2)] using electron microscopy. In freeze-fracture replicas of quick-frozen samples, PtdIns(3,5)P(2) was labeled specifically using recombinant ATG18 tagged with glutathione S-transferase and 4×FL...
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| Publicado no: | Commun Integr Biol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4857783/ https://ncbi.nlm.nih.gov/pubmed/27195064 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/19420889.2016.1145319 |
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