An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology

Transmission electron microscopy (EM) is a versatile technique that can be used to image biological specimens ranging from intact eukaryotic cells to individual proteins >150 kDa. There are several strategies for preparing samples for imaging by EM, including negative staining and cryogenic freez...

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Udgivet i:Methods
Main Authors: Thompson, Rebecca F., Walker, Matt, Siebert, C. Alistair, Muench, Stephen P., Ranson, Neil A.
Format: Artigo
Sprog:Inglês
Udgivet: Academic Press 2016
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4854231/
https://ncbi.nlm.nih.gov/pubmed/26931652
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2016.02.017
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