Optimized Protocol of Methanol Treatment for Immunofluorescent Staining in Fixed Brain Slices

We optimized methanol treatment in paraformaldehyde-fixed slices for immunofluorescent staining of ependymal basal bodies in brain ventricles. As 100% methanol induced severe deformations to the slices (including rolling and folding over), we tried to decrease methanol concentration. We found that 3...

Descripció completa

Guardat en:
Dades bibliogràfiques
Publicat a:Appl Immunohistochem Mol Morphol
Autors principals: Yuan, Feng, Xiong, Guoxiang, Cohen, Noam A., Cohen, Akiva S.
Format: Artigo
Idioma:Inglês
Publicat: Lippincott Williams & Wilkins 2017
Matèries:
Research Articles
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4848166/
https://ncbi.nlm.nih.gov/pubmed/26509907
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1097/PAI.0000000000000293
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!

Ítems similars