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Optimized Protocol of Methanol Treatment for Immunofluorescent Staining in Fixed Brain Slices
We optimized methanol treatment in paraformaldehyde-fixed slices for immunofluorescent staining of ependymal basal bodies in brain ventricles. As 100% methanol induced severe deformations to the slices (including rolling and folding over), we tried to decrease methanol concentration. We found that 3...
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| Publicado no: | Appl Immunohistochem Mol Morphol |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Lippincott Williams & Wilkins
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4848166/ https://ncbi.nlm.nih.gov/pubmed/26509907 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1097/PAI.0000000000000293 |
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