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Validation of Metabolic Alterations in Microscale Cell Culture Lysates Using Hydrophilic Interaction Liquid Chromatography (HILIC)-Tandem Mass Spectrometry-Based Metabolomics

By standard convention, in order to increase the efficacy of metabolite detection from cell culture lysates, metabolite extracts from a large quantity of cells are utilized for multiple reaction monitoring-based metabolomic studies. Metabolomics from a small number of cell extracts offers a potentia...

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Détails bibliographiques
Publié dans:PLoS One
Auteurs principaux: Gunda, Venugopal, Yu, Fang, Singh, Pankaj K.
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4847783/
https://ncbi.nlm.nih.gov/pubmed/27120458
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0154416
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