Isolation of human monoclonal antibodies from peripheral blood B cells

Isolation of monoclonal antibodies is an important technique for understanding the specificities and characteristics of antibodies that underlie the humoral immune response to a given antigen. Here we describe a technique for isolating monoclonal antibodies from human peripheral blood mononuclear ce...

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Publicado no:Nat Protoc
Main Authors: Huang, Jinghe, Doria-Rose, Nicole A, Longo, Nancy S, Laub, Leo, Lin, Chien-Li, Turk, Ellen, Kang, Byong H, Migueles, Stephen A, Bailer, Robert T, Mascola, John R, Connors, Mark
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4844175/
https://ncbi.nlm.nih.gov/pubmed/24030440
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.117
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