Protein affinity chromatography with purified yeast DNA polymerase alpha detects proteins that bind to DNA polymerase.
We have overexpressed the POL1 gene of the yeast Saccharomyces cerevisiae and purified the resulting DNA polymerase alpha polypeptide in an apparently intact form. We attached the purified DNA polymerase covalently to an agarose matrix and used this matrix to chromatograph extracts prepared from yea...
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| Yayımlandı: | Proc Natl Acad Sci U S A |
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| Asıl Yazarlar: | , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
National Academy of Sciences
1992
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48432/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1741381/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.4.1276 |
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