Fluorescence lifetime imaging of free and protein-bound NADH.

We introduce a methodology, fluorescence lifetime imaging (FLIM), in which the contrast depends on the fluorescence lifetime at each point in a two-dimensional image and not on the local concentration and/or intensity of the fluorophore. We used FLIM to create lifetime images of NADH when free in so...

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Bibliografiske detaljer
Main Authors: Lakowicz, J R, Szmacinski, H, Nowaczyk, K, Johnson, M L
Format: Artigo
Sprog:Inglês
Udgivet: 1992
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC48431/
https://ncbi.nlm.nih.gov/pubmed/1741380
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