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Fluorescence lifetime imaging of intracellular calcium in COS cells using Quin–2

We describe the first fluorescence lifetime images of cells. To demonstrate this new capability we measured Intracellular Images of Ca(2+) in COS cells based on the Ca(2+)-dependent fluorescence lifetime of Quin–2. Apparent fluorescence lifetimes were measured by the phase-modulation method using a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Cell Calcium
Hauptverfasser: LAKOWICZ, J.R., SZMACINSKI, H., NOWACZYK, K., LEDERER, W.J., KIRBY, M.S., JOHNSON, M.L.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1994
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6906927/
https://ncbi.nlm.nih.gov/pubmed/8149407
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/0143-4160(94)90100-7
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