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Fluorescence lifetime imaging of intracellular calcium in COS cells using Quin–2

We describe the first fluorescence lifetime images of cells. To demonstrate this new capability we measured Intracellular Images of Ca(2+) in COS cells based on the Ca(2+)-dependent fluorescence lifetime of Quin–2. Apparent fluorescence lifetimes were measured by the phase-modulation method using a...

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Détails bibliographiques
Publié dans:Cell Calcium
Auteurs principaux: LAKOWICZ, J.R., SZMACINSKI, H., NOWACZYK, K., LEDERER, W.J., KIRBY, M.S., JOHNSON, M.L.
Format: Artigo
Langue:Inglês
Publié: 1994
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6906927/
https://ncbi.nlm.nih.gov/pubmed/8149407
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/0143-4160(94)90100-7
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