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Decontamination of 16S rRNA gene amplicon sequence datasets based on bacterial load assessment by qPCR
BACKGROUND: Identification of unexpected taxa in 16S rRNA surveys of low-density microbiota, diluted mock communities and cultures demonstrated that a variable fraction of sequence reads originated from exogenous DNA. The sources of these contaminants are reagents used in DNA extraction, PCR, and ne...
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| Publicat a: | BMC Microbiol |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
BioMed Central
2016
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4842273/ https://ncbi.nlm.nih.gov/pubmed/27107811 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12866-016-0689-4 |
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