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A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences
Chemical synthesis of DNA sequences provides a powerful tool for modifying genes and for studying gene function, structure and expression. Here, we report a simple, high-fidelity and cost-effective PCR-based two-step DNA synthesis (PTDS) method for synthesis of long segments of DNA. The method invol...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2004
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC484193/ https://ncbi.nlm.nih.gov/pubmed/15240836 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh094 |
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