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Reverse Sanger sequencing of RNA by MALDI-TOF mass spectrometry after solid phase purification

Several DNA/RNA sequencing strategies have been developed using matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). In the reverse Sanger sequencing approach α-thiophosphate-containing NTPs are employed. Sequencing ladders are produced by the subsequent exonuclease cleavage, wh...

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Bibliografische gegevens
Hoofdauteurs: Spottke, Beatrice, Gross, Julia, Galla, Hans-Joachim, Hillenkamp, Franz
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2004
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC484192/
https://ncbi.nlm.nih.gov/pubmed/15240835
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh089
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