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Reverse Sanger sequencing of RNA by MALDI-TOF mass spectrometry after solid phase purification
Several DNA/RNA sequencing strategies have been developed using matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). In the reverse Sanger sequencing approach α-thiophosphate-containing NTPs are employed. Sequencing ladders are produced by the subsequent exonuclease cleavage, wh...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC484192/ https://ncbi.nlm.nih.gov/pubmed/15240835 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh089 |
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