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Characterization of Membrane Protein Interactions in Plasma Membrane Derived Vesicles with Quantitative Imaging FRET

Here we describe an experimental tool, termed Quantitative Imaging Förster Resonance Energy Transfer (QI-FRET), which enables the quantitative characterization of membrane protein interactions. The QI-FRET methodology allows us to acquire binding curves and calculate association constants for comple...

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Detalles Bibliográficos
Publicado en:Acc Chem Res
Autores principales: Sarabipour, Sarvenaz, Del Piccolo, Nuala, Hristova, Kalina
Formato: Artigo
Lenguaje:Inglês
Publicado: 2015
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4841635/
https://ncbi.nlm.nih.gov/pubmed/26244699
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.accounts.5b00238
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