Diaphorase Coupling Protocols for Red-Shifting Dehydrogenase Assays

Dehydrogenases are an important target for the development of cancer therapeutics. Dehydrogenases either produce or consume NAD(P)H, which is fluorescent but at a wavelength where many compounds found in chemical libraries are also fluorescent. By coupling dehydrogenases to diaphorase, which utilize...

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Bibliografski detalji
Izdano u:Assay Drug Dev Technol
Glavni autori: Davis, Mindy I., Shen, Min, Simeonov, Anton, Hall, Matthew D.
Format: Artigo
Jezik:Inglês
Izdano: Mary Ann Liebert, Inc. 2016
Teme:
Technical Brief
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4840998/
https://ncbi.nlm.nih.gov/pubmed/27078681
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/adt.2016.706
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