Site-specific quantification of lysine acetylation in the N-terminal tail of histone H4 using a double-labelling, targeted UHPLC MS/MS approach

We developed a targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the site-specific quantification of lysine acetylation in the N-terminal region of histone H4 by combining chemical derivatization at the protein and peptide levels with digestion using chymotrypsin and tryp...

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Detalhes bibliográficos
Publicado no:Anal Bioanal Chem
Main Authors: D’Urzo, Annalisa, Boichenko, Alexander P., van den Bosch, Thea, Hermans, Jos, Dekker, Frank, Andrisano, Vincenza, Bischoff, Rainer
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Berlin Heidelberg 2016
Assuntos:
Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4837199/
https://ncbi.nlm.nih.gov/pubmed/26968571
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-016-9431-1
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