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How does fluorescent labeling affect the binding kinetics of proteins with intact cells?

Fluorescent labeling is a mainstream technology for detecting molecular binding. Despite the importance, few studies have been devoted to quantitatively examine the effect of labeling on the molecular binding processes. Here we present a quantitative study on the binding kinetics of fluorescent-labe...

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Detalhes bibliográficos
Publicado no:Biosens Bioelectron
Main Authors: Yin, Linliang, Wang, Wei, Wang, Shaopeng, Zhang, Fenni, Zhang, Shengtao, Tao, Nongjian
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4836836/
https://ncbi.nlm.nih.gov/pubmed/25486538
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bios.2014.11.036
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