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Design and Development of High Bioluminescent Resonance Energy Transfer Efficiency Hybrid-Imaging Constructs

Here we describe the design and construction of an imaging construct with high bioluminescent resonance energy transfer (BRET) efficiency that is comprised of multiple quantum dots (QDs, λ(em) 655 nm) self-assembled onto a bioluminescent protein, Renilla luciferase (Rluc). This is facilitated by the...

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Detalhes bibliográficos
Publicado no:Anal Biochem
Main Authors: Kumar, Manoj, Kovalski, Letícia, Broyles, David, Hunt, Eric A., Daftarian, Pirouz, Diciki, Emre, Daunert, Sylvia, Deo, Sapna K.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4829111/
https://ncbi.nlm.nih.gov/pubmed/26772160
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.11.028
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