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Design and Development of High Bioluminescent Resonance Energy Transfer Efficiency Hybrid-Imaging Constructs

Here we describe the design and construction of an imaging construct with high bioluminescent resonance energy transfer (BRET) efficiency that is comprised of multiple quantum dots (QDs, λ(em) 655 nm) self-assembled onto a bioluminescent protein, Renilla luciferase (Rluc). This is facilitated by the...

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Bibliografski detalji
Izdano u:Anal Biochem
Glavni autori: Kumar, Manoj, Kovalski, Letícia, Broyles, David, Hunt, Eric A., Daftarian, Pirouz, Diciki, Emre, Daunert, Sylvia, Deo, Sapna K.
Format: Artigo
Jezik:Inglês
Izdano: 2016
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4829111/
https://ncbi.nlm.nih.gov/pubmed/26772160
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.11.028
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