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Design and Development of High Bioluminescent Resonance Energy Transfer Efficiency Hybrid-Imaging Constructs

Here we describe the design and construction of an imaging construct with high bioluminescent resonance energy transfer (BRET) efficiency that is comprised of multiple quantum dots (QDs, λ(em) 655 nm) self-assembled onto a bioluminescent protein, Renilla luciferase (Rluc). This is facilitated by the...

Полное описание

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Библиографические подробности
Опубликовано в: :Anal Biochem
Главные авторы: Kumar, Manoj, Kovalski, Letícia, Broyles, David, Hunt, Eric A., Daftarian, Pirouz, Diciki, Emre, Daunert, Sylvia, Deo, Sapna K.
Формат: Artigo
Язык:Inglês
Опубликовано: 2016
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4829111/
https://ncbi.nlm.nih.gov/pubmed/26772160
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.11.028
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