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Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

RNA-mediated knockdowns are widely used to control gene expression. This versatile family of techniques makes use of short RNA (sRNA) that can be synthesized with any sequence and designed to complement any gene targeted for silencing. Because sRNA constructs can be introduced to many cell types dir...

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Detalles Bibliográficos
Publicado en:J Vis Exp
Main Authors: Bernheim, Aude G., Libis, Vincent K., Lindner, Ariel B., Wintermute, Edwin H.
Formato: Artigo
Idioma:Inglês
Publicado: MyJove Corporation 2016
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4829038/
https://ncbi.nlm.nih.gov/pubmed/27023729
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53618
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