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Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

RNA-mediated knockdowns are widely used to control gene expression. This versatile family of techniques makes use of short RNA (sRNA) that can be synthesized with any sequence and designed to complement any gene targeted for silencing. Because sRNA constructs can be introduced to many cell types dir...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Bernheim, Aude G., Libis, Vincent K., Lindner, Ariel B., Wintermute, Edwin H.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4829038/
https://ncbi.nlm.nih.gov/pubmed/27023729
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53618
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