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A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture
Culturing primary hippocampal neurons in vitro facilitates mechanistic interrogation of many aspects of neuronal development. Dissociated embryonic hippocampal neurons can often grow successfully on glass coverslips at high density under serum-free conditions, but low density cultures typically requ...
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| 發表在: | J Vis Exp |
|---|---|
| Main Authors: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
MyJove Corporation
2016
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4828221/ https://ncbi.nlm.nih.gov/pubmed/27022758 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53797 |
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