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A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture
Culturing primary hippocampal neurons in vitro facilitates mechanistic interrogation of many aspects of neuronal development. Dissociated embryonic hippocampal neurons can often grow successfully on glass coverslips at high density under serum-free conditions, but low density cultures typically requ...
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| Published in: | J Vis Exp |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
MyJove Corporation
2016
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4828221/ https://ncbi.nlm.nih.gov/pubmed/27022758 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53797 |
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