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High-throughput Titration of Luciferase-expressing Recombinant Viruses

Standard plaque assays to determine infectious viral titers can be time consuming, are not amenable to a high volume of samples, and cannot be done with viruses that do not form plaques. As an alternative to plaque assays, we have developed a high-throughput titration method that allows for the simu...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: Garcia, Vanessa, Krishnan, Ramya, Davis, Colin, Batenchuk, Cory, Le Boeuf, Fabrice, Abdelbary, Hesham, Diallo, Jean-Simon
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2014
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4828131/
https://ncbi.nlm.nih.gov/pubmed/25285536
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51890
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