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Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Methods to detect and monitor mitochondrial outer membrane protein components in animal tissues are vital to study mitochondrial physiology and pathophysiology. This protocol describes a technique where mitochondria isolated from rodent tissue are immunolabeled and analyzed by flow cytometry. Mitoch...

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Foilsithe in:J Vis Exp
Main Authors: Pickles, Sarah, Arbour, Nathalie, Vande Velde, Christine
Formáid: Artigo
Teanga:Inglês
Foilsithe: MyJove Corporation 2014
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4828110/
https://ncbi.nlm.nih.gov/pubmed/25285411
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51887
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