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Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria
Methods to detect and monitor mitochondrial outer membrane protein components in animal tissues are vital to study mitochondrial physiology and pathophysiology. This protocol describes a technique where mitochondria isolated from rodent tissue are immunolabeled and analyzed by flow cytometry. Mitoch...
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| Publicado no: | J Vis Exp |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4828110/ https://ncbi.nlm.nih.gov/pubmed/25285411 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51887 |
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