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Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Methods to detect and monitor mitochondrial outer membrane protein components in animal tissues are vital to study mitochondrial physiology and pathophysiology. This protocol describes a technique where mitochondria isolated from rodent tissue are immunolabeled and analyzed by flow cytometry. Mitoch...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:J Vis Exp
Prif Awduron: Pickles, Sarah, Arbour, Nathalie, Vande Velde, Christine
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: MyJove Corporation 2014
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4828110/
https://ncbi.nlm.nih.gov/pubmed/25285411
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51887
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