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Real-time PCR probe optimization using design of experiments approach
Primer and probe sequence designs are among the most critical input factors in real-time polymerase chain reaction (PCR) assay optimization. In this study, we present the use of statistical design of experiments (DOE) approach as a general guideline for probe optimization and more specifically focus...
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| 出版年: | Biomol Detect Quantif |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Elsevier
2015
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4827641/ https://ncbi.nlm.nih.gov/pubmed/27077046 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bdq.2015.12.002 |
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