Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system.

An isothermal in vitro DNA amplification method was developed based upon the following sequence of reaction events. Restriction enzyme cleavage and subsequent heat denaturation of a DNA sample generates two single-stranded target DNA fragments (T1 and T2). Present in excess are two DNA amplification...

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Detalles Bibliográficos

Publicado en:	Proc Natl Acad Sci U S A
Autores principales:	Walker, G T, Little, M C, Nadeau, J G, Shank, D D
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	National Academy of Sciences 1992
Materias:	Research Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48243/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1309614/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.1.392
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