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Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system.

An isothermal in vitro DNA amplification method was developed based upon the following sequence of reaction events. Restriction enzyme cleavage and subsequent heat denaturation of a DNA sample generates two single-stranded target DNA fragments (T1 and T2). Present in excess are two DNA amplification...

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Dades bibliogràfiques
Publicat a:Proc Natl Acad Sci U S A
Autors principals: Walker, G T, Little, M C, Nadeau, J G, Shank, D D
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 1992
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48243/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1309614/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.1.392
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