Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system.
An isothermal in vitro DNA amplification method was developed based upon the following sequence of reaction events. Restriction enzyme cleavage and subsequent heat denaturation of a DNA sample generates two single-stranded target DNA fragments (T1 and T2). Present in excess are two DNA amplification...
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| Veröffentlicht in: | Proc Natl Acad Sci U S A |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
1992
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48243/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1309614/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.1.392 |
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