Native Immunogold Labeling of Cell Surface Proteins and Viral Glycoproteins for Cryo-Electron Microscopy and Cryo-Electron Tomography Applications

Numerous methods have been developed for immunogold labeling of thick, cryo-preserved biological specimens. However, most of the methods are permutations of chemical fixation and sample sectioning, which select and isolate the immunolabeled region of interest. We describe a method for combining immu...

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Veröffentlicht in:J Histochem Cytochem
Hauptverfasser: Yi, Hong, Strauss, Joshua D., Ke, Zunlong, Alonas, Eric, Dillard, Rebecca S., Hampton, Cheri M., Lamb, Kristen M., Hammonds, Jason E., Santangelo, Philip J., Spearman, Paul W., Wright, Elizabeth R.
Format: Artigo
Sprache:Inglês
Veröffentlicht: SAGE Publications 2015
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4823802/
https://ncbi.nlm.nih.gov/pubmed/26069287
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1369/0022155415593323
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