Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT

We present a plane-scanning RESOLFT [reversible saturable/switchable optical (fluorescence) transitions] light-sheet (LS) nanoscope, which fundamentally overcomes the diffraction barrier in the axial direction via confinement of the fluorescent molecular state to a sheet of subdiffraction thickness...

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Dades bibliogràfiques
Publicat a:Proc Natl Acad Sci U S A
Autors principals: Hoyer, Patrick, de Medeiros, Gustavo, Balázs, Bálint, Norlin, Nils, Besir, Christina, Hanne, Janina, Kräusslich, Hans-Georg, Engelhardt, Johann, Sahl, Steffen J., Hell, Stefan W., Hufnagel, Lars
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2016
Matèries:
Physical Sciences
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4822606/
https://ncbi.nlm.nih.gov/pubmed/26984498
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1522292113
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