Cleaning out the Litterbox of Proteomic Scientists’ Favorite Pet: Optimized Data Analysis Avoiding Trypsin Artifacts

[Image: see text] Chemically modified trypsin is a standard reagent in proteomics experiments but is usually not considered in database searches. Modification of trypsin is supposed to protect the protease against autolysis and the resulting loss of activity. Here, we show that modified trypsin is s...

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Bibliografiske detaljer
Udgivet i:J Proteome Res
Main Authors: Schittmayer, Matthias, Fritz, Katarina, Liesinger, Laura, Griss, Johannes, Birner-Gruenberger, Ruth
Format: Artigo
Sprog:Inglês
Udgivet: American Chemical Society 2016
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4820788/
https://ncbi.nlm.nih.gov/pubmed/26938934
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jproteome.5b01105
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