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High‐throughput identification of protein mutant stability computed from a double mutant fitness landscape

The effect of a mutation on protein stability is traditionally measured by genetic construction, expression, purification, and physical analysis using low‐throughput methods. This process is tedious and limits the number of mutants able to be examined in a single study. In contrast, functional fitne...

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Detalhes bibliográficos
Publicado no:Protein Sci
Main Authors: Wu, Nicholas C., Olson, C. Anders, Sun, Ren
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4815338/
https://ncbi.nlm.nih.gov/pubmed/26540565
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2840
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