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Design of Novel Relaxase Substrates Based on Rolling Circle Replicases for Bioconjugation to DNA Nanostructures

During bacterial conjugation and rolling circle replication, HUH endonucleases, respectively known as relaxases and replicases, form a covalent bond with ssDNA when they cleave their target sequence (nic site). Both protein families show structural similarity but limited amino acid identity. Moreove...

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Bibliografiske detaljer
Udgivet i:PLoS One
Main Authors: Sagredo, Sandra, de la Cruz, Fernando, Moncalián, Gabriel
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4814116/
https://ncbi.nlm.nih.gov/pubmed/27027740
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0152666
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