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Distribution of AAV8 particles in cell lysates and culture media changes with time and is dependent on the recombinant vector

With clinical trials ongoing, efficient clinical production of adeno-associated virus (AAV) to treat large numbers of patients remains a challenge. We compared distribution of AAV8 packaged with Factor VIII (FVIII) in cell culture media and lysates on days 3, 5, 6, and 7 post-transfection and found...

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Detalhes bibliográficos
Publicado no:Mol Ther Methods Clin Dev
Main Authors: Piras, Bryan A, Drury, Jason E, Morton, Christopher L, Spence, Yunyu, Lockey, Timothy D, Nathwani, Amit C, Davidoff, Andrew M, Meagher, Michael M
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4813606/
https://ncbi.nlm.nih.gov/pubmed/27069949
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mtm.2016.15
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