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Fluorescence Activated Cell Sorting of Rickettsia prowazekii-Infected Host Cells Based on Bacterial Burden and Early Detection of Fluorescent Rickettsial Transformants

Rickettsia prowazekii, the causative agent of epidemic typhus, is an obligate intracellular bacterium that replicates only within the cytosol of a eukaryotic host cell. Despite the barriers to genetic manipulation that such a life style creates, rickettsial mutants have been generated by transposon...

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Publicado en:PLoS One
Main Authors: Driskell, Lonnie O., Tucker, Aimee M., Woodard, Andrew, Wood, Raphael R., Wood, David O.
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2016
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4807063/
https://ncbi.nlm.nih.gov/pubmed/27010457
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0152365
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