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Rapid and Robust PCR-Based All-Recombinant Cloning Methodology
We report here a PCR-based cloning methodology that requires no post-PCR modifications such as restriction digestion and phosphorylation of the amplified DNA. The advantage of the present method is that it yields only recombinant clones thus eliminating the need for screening. Two DNA amplification...
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| Udgivet i: | PLoS One |
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| Main Authors: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Public Library of Science
2016
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4805250/ https://ncbi.nlm.nih.gov/pubmed/27007922 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0152106 |
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