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Large-Scale Single-Guide RNA Library Construction and Use for Genetic Screens: CRISPR/Cas9-based Genetic Screens
The ability to systematically disrupt genes serves as a powerful tool for understanding their function. The programmable Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system enables efficient targeting of large numbers of genes through the use of...
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| Veröffentlicht in: | Cold Spring Harb Protoc |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2016
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4804892/ https://ncbi.nlm.nih.gov/pubmed/26933254 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.top086892 |
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