Defined Essential 8™ Medium and Vitronectin Efficiently Support Scalable Xeno-Free Expansion of Human Induced Pluripotent Stem Cells in Stirred Microcarrier Culture Systems

Human induced pluripotent stem (hiPS) cell culture using Essential 8™ xeno-free medium and the defined xeno-free matrix vitronectin was successfully implemented under adherent conditions. This matrix was able to support hiPS cell expansion either in coated plates or on polystyrene-coated microcarrie...

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Bibliographic Details
Published in:PLoS One
Main Authors: Badenes, Sara M., Fernandes, Tiago G., Cordeiro, Cláudia S. M., Boucher, Shayne, Kuninger, David, Vemuri, Mohan C., Diogo, Maria Margarida, Cabral, Joaquim M. S.
Format: Artigo
Language:Inglês
Published: Public Library of Science 2016
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4801338/
https://ncbi.nlm.nih.gov/pubmed/26999816
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0151264
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