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Budding Yeast Silencing Complexes and Regulation of Sir2 Activity by Protein-Protein Interactions

Gene silencing in the budding yeast Saccharomyces cerevisiae requires the enzymatic activity of the Sir2 protein, a highly conserved NAD-dependent deacetylase. In order to study the activity of native Sir2, we purified and characterized two budding yeast Sir2 complexes: the Sir2/Sir4 complex, which...

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Bibliografiske detaljer
Main Authors: Tanny, Jason C., Kirkpatrick, Donald S., Gerber, Scott A., Gygi, Steven P., Moazed, Danesh
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2004
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC479720/
https://ncbi.nlm.nih.gov/pubmed/15282295
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.24.16.6931-6946.2004
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