Implications of multiple freeze-thawing on respiratory samples for culture-independent analyses()

BACKGROUND: Best practice when performing culture-independent microbiological analysis of sputum samples involves their rapid freezing and storage at −80 °C. However, accessing biobanked collections can mean that material has been passed through repeated freeze–thaw cycles. The aim of this study was...

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Detalhes bibliográficos
Publicado no:J Cyst Fibros
Main Authors: Cuthbertson, Leah, Rogers, Geraint B., Walker, Alan W., Oliver, Anna, Hoffman, Lucas R., Carroll, Mary P., Parkhill, Julian, Bruce, Kenneth D., van der Gast, Christopher J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4793934/
https://ncbi.nlm.nih.gov/pubmed/25459563
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jcf.2014.10.004
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