A Versatile Assay for High-Throughput Gene Expression Profiling on Universal Array Matrices

We report a flexible, sensitive, and quantitative gene-expression profiling system for assaying more than 400 genes, with three probes per gene, for 96 samples in parallel. The cDNA-mediated annealing, selection, extension and ligation (DASL) assay targets specific transcripts, using oligonucleotide...

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Veröffentlicht in:Genome Res
Hauptverfasser: Fan, Jian-Bing, Yeakley, Joanne M., Bibikova, Marina, Chudin, Eugene, Wickham, Eliza, Chen, Jing, Doucet, Dennis, Rigault, Philippe, Zhang, Baohong, Shen, Richard, McBride, Celeste, Li, Hai-Ri, Fu, Xiang-Dong, Oliphant, Arnold, Barker, David L., Chee, Mark S.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Cold Spring Harbor Laboratory Press 2004
Schlagworte:
Methods
Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC479115/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15123585/
https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.2167504
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