A Versatile Assay for High-Throughput Gene Expression Profiling on Universal Array Matrices

We report a flexible, sensitive, and quantitative gene-expression profiling system for assaying more than 400 genes, with three probes per gene, for 96 samples in parallel. The cDNA-mediated annealing, selection, extension and ligation (DASL) assay targets specific transcripts, using oligonucleotide...

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Detalles Bibliográficos
Autores principales: Fan, Jian-Bing, Yeakley, Joanne M., Bibikova, Marina, Chudin, Eugene, Wickham, Eliza, Chen, Jing, Doucet, Dennis, Rigault, Philippe, Zhang, Baohong, Shen, Richard, McBride, Celeste, Li, Hai-Ri, Fu, Xiang-Dong, Oliphant, Arnold, Barker, David L., Chee, Mark S.
Formato: Artigo
Lenguaje:Inglês
Publicado: Cold Spring Harbor Laboratory Press 2004
Materias:
Methods
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC479115/
https://ncbi.nlm.nih.gov/pubmed/15123585
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.2167504
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