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Application of linker technique to trap transiently interacting protein complexes for structural studies

Protein-protein interactions are key events controlling several biological processes. We have developed and employed a method to trap transiently interacting protein complexes for structural studies using glycine-rich linkers to fuse interacting partners, one of which is unstructured. Initial steps...

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Bibliographische Detailangaben
Veröffentlicht in:J Biol Methods
Hauptverfasser: Chichili, Vishnu Priyanka Reddy, Kumar, Veerendra, Sivaraman, J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: jbm 2016
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4789767/
https://ncbi.nlm.nih.gov/pubmed/26985443
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14440/jbm.2016.81
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