Ultrasensitive Time-Resolved Fluoroimmunoassay for Saikosaponin a in Chaihu (Bupleuri Radix)

The aim of this study is to establish a time-resolved fluoroimmunoassay (TRFIA) system for quantitative analysis of saikosaponin a (SSa) in the crude drug of Chaihu (Bupleuri Radix). A 96-well microplate coated with rabbit anti-mouse IgG was incubated with the methanol extracts of Chaihu samples and...

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Bibliographic Details
Published in:PLoS One
Main Authors: Chao, Zhi, Cui, Qian, Tian, Enwei, Zeng, Weiping, Cai, Xuan, Li, Xiaolei, Tanaka, Hiroyuki, Shoyama, Yukihiro, Wu, Yingsong
Format: Artigo
Language:Inglês
Published: Public Library of Science 2016
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4788217/
https://ncbi.nlm.nih.gov/pubmed/26968034
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0151032
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