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Global analysis of RNA cleavage by 5′-hydroxyl RNA sequencing

RNA cleavage by some endoribonucleases and self-cleaving ribozymes produces RNA fragments with 5′-hydroxyl (5′-OH) and 2′,3′-cyclic phosphate termini. To identify 5′-OH RNA fragments produced by these cleavage events, we exploited the unique ligation mechanism of Escherichia coli RtcB RNA ligase to...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Peach, Sally E., York, Kerri, Hesselberth, Jay R.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4787814/
https://ncbi.nlm.nih.gov/pubmed/26001965
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv536
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