DNA sequencing by MALDI-TOF MS using alkali cleavage of RNA/DNA chimeras

Approaches developed for sequencing DNA with detection by mass spectrometry use strategies that deviate from the Sanger-type methods. Procedures demonstrated so far used the sequence specificity of RNA endonucleases, as unfortunately equivalent enzymes for DNA do not exist and therefore require tran...

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Autors principals: Mauger, Florence, Bauer, Keith, Calloway, Cassandra D., Semhoun, Jérémy, Nishimoto, Tetsuya, Myers, Thomas W., Gelfand, David H., Gut, Ivo G.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2007
Matèries:
Methods Online
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1885642/
https://ncbi.nlm.nih.gov/pubmed/17426131
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm056
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