Silent IL2RG Gene Editing in Human Pluripotent Stem Cells

Many applications of pluripotent stem cells (PSCs) require efficient editing of silent chromosomal genes. Here, we show that a major limitation in isolating edited clones is silencing of the selectable marker cassette after homologous recombination and that this can be overcome by using a ubiquitous...

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Enregistré dans:
Détails bibliographiques
Publié dans:Mol Ther
Auteurs principaux: Li, Li B, Ma, Chao, Awong, Geneve, Kennedy, Marion, Gornalusse, German, Keller, Gordon, Kaufman, Dan S, Russell, David W
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group 2016
Sujets:
Original Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4786911/
https://ncbi.nlm.nih.gov/pubmed/26444081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mt.2015.190
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