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Temperature Sensitivity Conferred by ligA Alleles from Psychrophilic Bacteria upon Substitution in Mesophilic Bacteria and a Yeast Species

We have assembled a collection of 13 psychrophilic ligA alleles that can serve as genetic elements for engineering mesophiles to a temperature-sensitive (TS) phenotype. When these ligA alleles were substituted into Francisella novicida, they conferred a TS phenotype with restrictive temperatures bet...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Pankowski, Jarosław A., Puckett, Stephanie M., Nano, Francis E.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4784036/
https://ncbi.nlm.nih.gov/pubmed/26773080
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03890-15
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